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The purpose of this document is to provide interim guidance to laboratories and stakeholders involved in laboratory testing of COVID-19 virus.
- Read the document66顺彩票app (updated 2 March 2020)
The purpose of this document is to provide interim guidance on laboratory biosafety related to the COVID-19 virus to laboratories and stakeholders involved in COVID-19 virus laboratory work. This also includes the packaging and shipment requirements for sending specimens to WHO reference laboratories providing confirmatory testing for COVID-19.
Several assays that detect the 2019-nCoV have been and are currently under development, both in-house and commercially. Some assays may detect only the novel virus and some may also detect other strains (e.g. SARS-CoV) that are genetically similar.
In-house developed molecular assays
Some groups shared their protocols which can be accessed below (Summary table and link to the protocols). In some cases, the groups will be willing to send reagents or reagent mixes prepared in their laboratories, with or without associated fees. It is strongly recommended to contact the developer if you need further assistance. The list is not exhaustive and is being updated regularly.
Summary table of available protocols
|China||China CDC||ORF1ab and N|
|Germany||Charité||RdRP, E, N|
|Hong Kong SAR||HKU||ORF1b-nsp14, N|
|Japan||National Institute of Infectious Diseases, Department of Virology III||Pancorona and multiple targets, Spike protein|
|Thailand||National Institute of Health||N|
|US||US CDC||Three targets in N gene|
|France||Institut Pasteur, Paris||Two targets in RdRP|
(24 January 2020)
PCR and sequencing protocol for 2019-nCoV - Department of Medical Sciences, Ministry of Public Health, Thailand66顺彩票app (Updated 28 January 2020)
US CDC Real-Time RT-PCR Panel for Detection 2019-Novel Coronavirus (28 January 2020)
US CDC panel primer and probes– U.S. CDC, USA66顺彩票app (28 January 2020)
Real-time RT-PCR assays for the detection of SARS-CoV-2 Institut Pasteur, Paris 66顺彩票app(2 March 2020)
Disclaimer: The order on the list is by country of the hosting institution and does not imply any preference of WHO. Neither the names of vendors or manufacturers included in the protocols are preferred/endorsed by WHO. The protocols have not yet been validated through a WHO process.
Assumptions: Most procedures assume a basic familiarity with PCR/RT-PCR assays.
Safety Information: Specimen processing should be performed in accordance with pertaining national biological safety regulations and following the recommended WHO guidelines on biosafety and biosecurity.
Protocol use limitations: Optional clinical specimens for testing has not yet been validated.
Countries who have no testing capacity and national COVID-19 laboratories with limited experience on COVID-19 virus testing are encouraged to send the first five positives and the first ten negative COVID-19 samples to WHO reference laboratories providing confirmatory testing for COVID-19.
Current list of WHO reference laboratories providing confirmatory testing for COVID-1966顺彩票app (Updated 2 March 2020)
Booking form for national laboratories (Updated 11 March 2020)
Contact details of WHO reference laboratories providing confirmatory testing for COVID-19 can be obtained from the respective regional WHO offices.